PFA-100 System

The PFA-100^® System measures the complex process of primary hemostasis and aids in the rapid detection of platelet dysfunction. It is the first commercially available in vitro testing system to incorporate high-shear flow in which the process of platelet adhesion and aggregation following a vascular injury is simulated in-vitro.

The PFA-100 System provides automated assessment for inherited, acquired, or drug-induced platelet dysfunction.

Helps detect inherited, acquired and drug-induced platelet dysfunction
Screening test for patients with impaired primary hemostasis, such as von Willebrand disease¹
Verifies efficacy of desmopressin acetate (DDAVP) therapy in pre-surgical patients²
Assesses the platelet dysfunction due to the effect of Aspirin
Evaluates platelet dysfunction in children over 1 year of age³
Evaluates platelet dysfunction in multiple clinical settings, such as presurgical screening, high-risk pregnancy and menorrhagia

Features & Benefits

The PFA-100 System has become the new standard for detection of platelet dysfunction. The PFA-100 Analyzer quickly and easily measures platelet plug formation in a small, whole blood sample (<1 mL) and reports a ”Closure Time” in 5-8 minutes. It is a unique system that challenges platelets under high shear flow conditions similar to the physiologic environment in which platelets normally function. It detects inherited-, acquired- or induced- platelet dysfunction by uniquely simulating, in vitro, the function of platelets in Primary Hemostasis

A Model of Efficiency:

Accurate detection of inherited, acquired or induced platelet dysfunction under high-shear stress
Sensitive to quantitative and qualitative abnormalities of VWD in both plasma and platelets
Simple, rapid and precise assessment of platelet function

Test Menu:

Collagen/Epinephrine and Collagen/ADP test cartridges

Features:

Easy to use - self-contained disposable test cartridges
Fast - delivers quantitative results within 5 to 8 minutes
Cost savings - minimal sample manipulation and maintenance required

Technical Specifications

PFA-100 System

Test System
Principle	High-shear force dynamic flow system
Measuring channel	Single measuring channel
Incubation category	Two incubation wells
Incubation temperature	37.9 °C ± 1.0 °C
Incubation warm up-time	<15 minutes (room to operating temperature)

Sample Handling
Type of sample	Citrated whole blood
Sample mechanism	Manual pipette dispensing (800 μL)
Barcode identification	Optional external barcode reader for sample identification

Reagent Handling
Type	Single disposable self-contained test cartridge
Reagent recognition	Positive optical coding reagent cartridge identification
Additional reagent	Trigger solution

Throughput
Single test	Col/EPI or Col/ADP: 6 tests/hour
Duplicate test	Col/EPI or Col/ADP: 10 tests/hour

Software
Interface	LCD display
Host connection	Unidirectional, external PC RS-232 25 pin D port
Patient ID	Maximum 12 numeric characters
Stored control results	Last 50 results
Stored patient results	Last 20 results
Software update	PCMCIA Card

Printer
Printer	Built-in dot matrix printer, sound level printing 77 dBA
Type	Ribbon Epson #ERC-22 black or #ERC-09 purple
Printer paper	58 mm wide paper roll

Power Supply
Operating voltage	100 – 130 V / 60 Hz, 200 – 250 V / 50 Hz
Mains frequency	50 – 60 Hz
Current (maximum)	1.5 A @ 100 V, 0.75 A @ 200 V
Power consumption	110 W (maximum)
Fuse	Sloblo, 250 V, 1.5 A
Note	Main supply voltage should not exceed recommended ranges

Environmental Conditions
Operating temperature	18 - 29 °C
Non-operating storage	-29 - 50 °C
Relative humidity	20 - 90 %
Altitude (maximum)	2000 meters
Operating humidity	20 - 90 % RH
Non-operating storage humidity	0 - 95 % RH
Heat emission	94 kcal/h (375 BTU/h)

Dimensions
Dimensions	384 mm x 229 mm x 360 mm (W x D x H)
Weight	10.9 kg

Remarks
The PFA-100 System is available for sale in the US and complies to the IVD Directive 98/79/EC.

Ordering Information

Catalog No.	Product Description	Quantity
B4170-00	PFA-100 Analyzer	1 unit
B4170-20	Dade^® PFA-100 Collagen/EPI Test Cartridges	1 x 20 pieces
B4170-21	Dade PFA-100 Collagen/ADP Test Cartridges	1 x 20 pieces
B4170-50	Dade PFA-100 Trigger Solution	3 x 11 mL

Assays

Platelet Function

Collagen
Collagen/ADP

Download the assay menu for PFA-100^®System as PDF (pdf) 0.1 MB

Services & Support

Education & Training

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1 Koscielny J, Ziemer S, Radtke H, et al. A practical concept for preoperative identification of patients with impaired primary hemostasis. Clin Appl Thromb Hemost. 2004;10:195-204.

2 Koscielny J, von Tempelhoff GF, Ziemer S, et al. A practical concept for preoperative management of patients with impaired primary hemostasis. Clin Appl Thromb Hemost. 2004;10:155-66.

3 Lippi G, Manzato F, Franchini M, et al. Establishment of reference values for the PFA-100 platelet function analyzer in pediatrics. Clin Exp Med. 2001;1:69-70.

PFA-100 System

Features & Benefits

Technical Specifications

PFA-100 System

Ordering Information

Assays

Platelet Function

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